Krushynska O.A. Copper and iron chelates adsorbed on silica as solid-phase redox reagents for the determination of the organic reductants. – Manuscript.

The thesis for a Candidate’s degree in chemical science by speciality 02.00.02 – analytical chemistry. – Kyiv National Taras Shevchenko University, Kyiv, 2005.

The dissertation is devoted to the elaboration of the solid-phase redox reagents on the base of copper and and iron chelates immobilized on silica and the investigation of their heterophase redox-reactions with the organic reductants. The conditions of bis-bathocuproine-copper(II), phenanthroline-iron(III) and tetrabenzotetraazacyclohexadecine complexes immobilization on silica have been optimized. Solid-phase spectrophotometric and test methods of ascorbic acid and analgin determination in pharmaceuticals using solid-phase redox reagents have been elaborated. A solid-phase redox reagent for the test evaluation of phytoceuticals and herbal products antioxidant activity was proposed. A spectrophotometric method was elaborated, that allowed to determine selectively the content of caffeic acid and its derivatives in echinacea medicines; the correlation between this value and the antioxidant power of the phytoceuticals was studied at first. By the investigations of the correlations between the antioxidant activity and the content of the most important bioactive compounds of echinacea medicines, teas and red wines the availability of the test method for the evaluation of this integrated characteristic was confirmed, and the contribution of these groups of compounds to the total antioxidant activity of the herbal material was evaluated. Methodological approaches to the elaboration of the test-systems for the determination of individual antioxidants and the evaluation of the antioxidant activity of herbal products have been proposed.

Keywords: solid-phase reagents, chelate complexes, immobilization, solid-phase spectrophotometry, organic reductants, antioxidants, total antioxidant activity, pharmaceuticals, echinacea medicines, teas and red wines